Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.

نویسندگان

  • R M Coleman
  • L Pereira
  • P D Bailey
  • D Dondero
  • C Wickliffe
  • A J Nahmias
چکیده

We determined type-specific antibodies to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by an indirect enzyme-linked immunosorbent assay, using as antigens HSV-1 glycoprotein gC-1 and a HSV-2-specific polypeptide purified on affinity columns of monoclonal antibodies. All sera were initially screened for HSV antibodies by the enzyme-linked immunosorbent assay with a pool of Triton X-100-extracted antigens of HSV-1- and HSV-2-infected HEp-2 cells. The titer of HSV antibodies was predicted from a linear regression curve based on the absorbance of the initial 1:50 serum dilution. The sensitivity and specificity of the screening assay and of the assay for type-specific antibodies were established.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 18 2  شماره 

صفحات  -

تاریخ انتشار 1983